MOTLALEPULA SWEETNESS GYAMBOE
NGS Research And Development Scientist
Johannesburg
Summary
Nationality: South Africa Profession: NGS Research and Development Scientist PERSONAL PROFILE I hold an MSc. Medical Microbiology a certificate in Next Generation Sequencing Bioinformatics. I have experience as a Molecular Biology tutor a Laboratory Medical Scientist. I am currently employed as an NGS Research and development Scientist. As an R&D Medical Scientist I coordinate Next generation sequencing projects. The above includes drafting proposals for funding, conducting evaluations of available SARS-CoV-2 & Mycobacterium tuberculosis NGS assays. The wet lab experience includes DNA/RNA extraction, cDNA synthesis, manual and automated library preparation and sequencing on different platforms such as Illumina MiSeq, Ion torrent and Oxford Nanopore platforms such as the MinION. I also possess analytical experience using command line based pipelines such as STATA, R, Galaxy and User interphase experience for most pipelines used to analyze SARS- CoV-2 fastq files.
Overview
5
5
years of professional experience
6
6
years of post-secondary education
Work History
NGS Research and Development Scientist
WITS Diagnostic Innovation Hub
Johannesrburg
07.2020 - Current
- Documented changes during experiments, noting unexpected issues arising during research activities.
- Conducted experiments in laboratory environment for product development purposes.
- Evaluated and implemented new NGS assays and technologies and laboratory techniques to improve quality and robustness of sample analysis.
- Performed troubleshooting and assisted in leading initiatives to solve challenging scientific problems with NGS assays or instrumentation.
- Read literature related to scientific area of expertise to remain current in knowledge.
- Served as technical liaison between multi-functional departments, suppliers and trade compliance groups for importing and exporting of business and technical data, classifications, licensing and controls.
Medical Laboratory Scientist
Ampath
Pretoria
11.2017 - 07.2020
- Performed pre-analytical, analytical and post-analytical processing procedures (DNA extraction, qPCR, PCR, analysis or results and result reporting).
- Maintained strict adherence to prescribed lab safety guidelines and operational instructions.
- Documented test results using computerized and manual transcription methods.
- Carefully handled and disposed of biological hazardous materials following strict safety procedures to avoid contamination.
- Conducted root cause analysis to enhance quality and laboratory processes.
Education
No Degree - Bioinformatics
H3ABionet
Johannesburg, South Africa 04.2001 -
Master of Science - Medical Microbiology
University of Pretoria
Pretoria, South Africa 01.2016 - 11.2017
Bachelor of Science - Medical Microbiology
University of Pretoria
Pretoria, South Africa 01.2015 - 12.2015
Bachelor of Science - Applied Microbiology
University of Pretoria
Pretoria, South Africa 01.2012 - 12.2014
Skills
Research and development Medical Scientist – Wits Diagnostic Innovation HubKnowledge of qualitative research methodologies (literature review, SOP writing and reviewPresentation of findings, project management), GCP & GCLP
Wet lab experience (DNA extraction, PCR, qPCR, Next generation sequencing methodologies
Analytical skills (R, STATA, Linux)IT skills (Word, Excel & PowerPoint)Medical Scientist – Ampath laboratoriesWet lab experience qPCR, library preparation & NGS, automated platforms for DNA extraction
Familiar with laboratory working systems- MEDITECH- & ICT (Microsoft Office & involved inLearned and improved tutoring skills (Interactive Presentation) and Communication skillsPresentations and meetings
Timeline
NGS Research and Development Scientist
WITS Diagnostic Innovation Hub
07.2020 - Current
Medical Laboratory Scientist
Ampath
11.2017 - 07.2020
Master of Science - Medical Microbiology
University of Pretoria
01.2016 - 11.2017
Bachelor of Science - Medical Microbiology
University of Pretoria
01.2015 - 12.2015
Bachelor of Science - Applied Microbiology
University of Pretoria
01.2012 - 12.2014
No Degree - Bioinformatics
H3ABionet
04.2001 -