Puseletso Constance Kumalo
Biotechnologist
Witsieshoek
Summary
A highly motivated and dedicated Master’s graduate in Applied Science (Biotechnology) with a strong foundation in molecular biology and microbiology. Eager to apply academic knowledge and laboratory skills in a dynamic work environment. Passionate about contributing to genomic research and bioinformatics training while gaining valuable hands-on experience. Committed to supporting a collaborative and inclusive work culture, Eager to contribute to the success of the team and further develop skills in molecular techniques and research dissemination
Overview
1
1
year of professional experience
Work History
Inservice Trainee
Institute for Water and Wastewater Technology, DUT
Durban
01.2017 - 12.2017
- Administrative work, research, and report writing (Microsoft Office applications: Excel, Word, PowerPoint, and Outlook).
- Conducted laboratory clean-up, autoclaving, and centrifugation, as well as bacterial media preparation, and collected samples.
- Extracted nucleic acids and performed amplicon/nucleic acid purification, cDNA synthesis, and gel electrophoresis.
- Prepared project presentations and reports to assist senior staff.
- Performed laboratory research work utilizing microbiological and molecular techniques, including bacterial culturing and identification, colony count, biochemical analysis, PCR, qPCR, ddPCR, and gene expression.
- Worked on a research project focusing on antibiotic-resistant gene analysis and microbial identification through amplification and gene expression.
Education
Master of Applied Science - Biotechnology
Durban University of Technology
Durban, South Africa 04.2001 -
Bachelor of Technology - Biotechnology
Durban University of Technology
Durban, South Africa 04.2001 -
National Diploma - Biotechnology
Durban University of Technology
Durban, South Africa 04.2001 -
National Senior Certificate -
Molapo Secondary School
Phuthaditjhaba, South Africa 04.2001 -
Skills
Excel, PowerPoint, Word, Outlook and Teams
Bioinformatics tools (R, Ubuntu, Qiime2, and KBase)
Team player and team building
Interpersonal and communication
Problem-solving and critical thinking,
Time management and multitasking
Accomplishments
- Molecular techniques and bioinformatics training
NRF-STINT project: SVI and DUT [29/04/2019 – 03/05/2019]
City: Durban | Country: South Africa - Discovering biological patterns from microbiome sequence data: a hands-on short course
University of Minnesota and DUT [08/08/2022 – 19/08/2022]
City: Durban | Country: South Africa - DIPLOMICS/Thermofisher R for Scientists course
DIPLOMICS/Thermofisher [05/09/2022 – 14/09/2022]
City: Durban | Country: South Africa - Bacterial whole genome sequencing analysis
Agricultural Research Council [07/11/2022 – 10/11/2023]
City: Pretoria - RNA-Seq Data Analysis Certificate
Agricultural Research Council [13/02/2023 – 17/02/2023]
City: Pretoria
Volunteer Experience
NKA'THUTO - KwaZulu Natal
03/12/2019 - Current
• Facilitates the workshop,
• Assisted students with their projects
• Mentor learners,
• Judging the learner's projects
Eskom expo for young scientists - KwaZulu Natal
03/10/2022 - 05/20/2022
- Judging the learners' • science project.
Timeline
Inservice Trainee
Institute for Water and Wastewater Technology, DUT
01.2017 - 12.2017
Master of Applied Science - Biotechnology
Durban University of Technology
04.2001 -
Bachelor of Technology - Biotechnology
Durban University of Technology
04.2001 -
National Diploma - Biotechnology
Durban University of Technology
04.2001 -
National Senior Certificate -
Molapo Secondary School
04.2001 -
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