TUMELO VINCENT RATOPOLA
Research Technician
Pretoria
Summary
Dynamic Research Technician with a proven track record at the Agricultural Research Council, skilled in advanced laboratory techniques and effective communication. Adept at supervising teams and enhancing operational efficiency, I excel in cultivating cell lines and preparing virus growth, ensuring meticulous attention to detail in all processes.
Overview
15
15
years of professional experience
Work History
Research Technician
Agricultural Research Council (ARC) - TADP
Onderstepoort, South Africa
07.2016 - Current
- Cultivation of BHK 21 C13 in T flask and monitoring of their cell growth
- Cultivation of BHK38E3 cells in 5 & 20 litres fermenters and roller bottles,
- Preparation of virus growth and cells infection with FMD virus
- Inactivation of FMDV using BEI
- Actively participated in lab meetings, sharing knowledge and expertise to foster a collaborative research atmosphere.
- Supervise and monitors daily operations of research assistants.
- Supervise the preparation of starting materials by the research assistants.
- Record the receival of raw materials in their respective Logs.
- Monitor the availability of stock items needed on weekly basis.
- Make stock requisition and purchasing for items needed using the SAGEX3 ER software.
- Ensure that autoclaves, LAFC, fridges and freezers, BSC, and washing machine are operational and notify their faulty to relevant department.
- Ensure that autoclaves, LAFC, fridges and freezers, BSC, and washing machine are operational and notify their faulty to relevant department.
- Ensure that incidents occurring are reported on time.
BSc Honours Student
Agricultural Research Council (ARC) - TADP
Onderstepoort, South Africa
08.2013 - 06.2017
- Cell culturing, passaging of BHK 38E3 cells,
- Preparation of virus growth,
- Cell counting,
- DNA extraction & purification,
- PCR, Gel electrophoresis, cloning
- Searched at library and in scholarly databases to locate necessary information for research projects.
Junior Volunteer
SAASTA - ARC (OVI)
Onderstepoort, South Africa
02.2013 - 06.2013
- Antimicrobial disk susceptibility testing, freeze drying, API 20E testing,
- DNA extraction, sample preparation, preparation and dilution of reference standards,
- HLPC preparations, LC-MS/MS preparations,
- Testing of aflatoxins, tetracyclines, in honey, bovine, milk and ovine
- Rotovapor use, pH meter calibration,
- Preparation of Buffers and mobile phases, sample sonication, centrifuge use, thermometer calibration.
Research Technician
South Africa Medical Research Council (SAMRC)
Pretoria, South Africa
11.2009 - 04.2012
- Research Technician & Community Advisory Board Member (C.A.B Secretary)
- HIV/AIDS Prevention, Intervention in bars & shebeens in rural areas of North West Province,
- Writing minutes for board and management committee,
- Circulating agenda & notice of members, coordinating & planning activities)
Education
BSc Honours In Medical Sciences In Microbiology - Medical Sciences
Sefako Makgatho Health Sciences University
Ga-Rankuwa, South Africa 04.2001 -
Diploma In Leadership & Management - Leadership
Shaw Academy
Dublin, Ireland 04.2001 -
Bachelor of Science - Natural Sciences: Chemistry And Microbiology
University Of South Africa
Pretoria, South Africa 04.2001 -
International Computer Driving Licence Certificate - Computer
University Of South Africa
Pretoria, South Africa 04.2001 -
Skills
- Verbal and written communication
- Adaptability and flexibility
- Effective communication
- Laboratory techniques
- Written and oral communication
- Organizational skills
- Team building
- Attention to detail
Additional Information
REFERENCES
- Ms Eileen Rich - Project Manager (Medical Research Council)
Contact details : (012) 339 8578
- Dr Faith Peta - Production Team Leader (ARC-TADP)
Contact details : (012) 529 9577
- Ms Virginia Mahlangu - Quality Manager (ARC-TADP)
Contact details : (012) 529 9576
Timeline
Research Technician
Agricultural Research Council (ARC) - TADP
07.2016 - Current
BSc Honours Student
Agricultural Research Council (ARC) - TADP
08.2013 - 06.2017
Junior Volunteer
SAASTA - ARC (OVI)
02.2013 - 06.2013
Research Technician
South Africa Medical Research Council (SAMRC)
11.2009 - 04.2012
BSc Honours In Medical Sciences In Microbiology - Medical Sciences
Sefako Makgatho Health Sciences University
04.2001 -
Diploma In Leadership & Management - Leadership
Shaw Academy
04.2001 -
Bachelor of Science - Natural Sciences: Chemistry And Microbiology
University Of South Africa
04.2001 -
International Computer Driving Licence Certificate - Computer
University Of South Africa
04.2001 -