Kovin Ashley Chetty

Encouraging and positive Personal Tutor with 10 years of experience instructing students needing help with various subjects at. Inquisitive professional offering student support, providing research-driven guidance and drastically improving academic performance. Diplomatic liaison between student and parent, communicating goals and giving regular progress reports.

• 2019, Special Training Course in qPCR/RT-qPCR and Next Generation Sequencing on the Illumina Platform at the Centre of Proteomic and Genomic Research (CPGR). cDNA synthesis, QC on the Agilent Bioanalyzer, Template, Primer Concentration and Annealing Temperature Optimization qPCRs on the Applied Biosystems Quantstudio 12KFlex Thermal Cycler. Data Output and Analysis. DNA Isolation, QC on the Qubit 4 Fluorometer, Library Preparation: Barcoding, Size Selection, End Over Hang Repair, 3'A-Tailing, Adaptor Ligation, Library Amplification. Library Pooling, Loading of Cartridges into Illumina NextSeq Machine
• 2014-2017, Techniques Learned and Performed During Doctoral Degree. Soybean and Maize cultivation and maintenance, RNA/DNA isolation, RT-PCR, Semi-Quantitative PCR, NATIVE-PAGE, SDS-PAGE Spectrophotometric enzyme assays. Molecular Cloning: I successively cloned a transformational cassette including promoter, GOI and terminator sequence into an Agrobacterium plasmid for overexpression. Primer design, bioinformatics, in silico analyses. Sequence alignment from Sanger sequence data. Restriction enzyme analyses of sequences.
• 2008-2012, Techniques Learned and Performed During Masters Degree. C2C12 Myotube Culture, Protein Extraction and Quantification, SDS-PAGE, Western Blotting. Chromatin Immunoprecipitation (ChIP) Assay. DNA Isolation. PCR
• 2006-2007, Techniques Learned and Performed During Honours Degree. Rodent Handling and Whole Body Dissection, Single Cell Gel Electrophoresis (SCGE or Comet) Assay, Routine Tissue Preparation for Histology, Immunohistochemistry, The Thiobarbituric Acid Reactive Substances (TBARS) Assay, Enzyme-Linked Immunosorbent Assay (ELISA), Cytotoxicity assays.

• 2011, Caffeine Induces Histone Hyper-acetylation at the MEF2A binding site on the Glut4 gene via p300 up regulation, 39th Physiological Society of South Africa (PSSA) Annual Conference, 08/01/11, Cape Town, South Africa
• 2009, Exercise & Type II Diabetes: How Chromatin Remodeling May Influence Glucose Transport, Cell Biology Mini Symposium 2009, 06/01/09, Cape Town, South Africa
• 2007, The effects of Sutherlandia Frutescens on mycotoxin treated rats, Pfizer/UKZN College of Health Science Research Symposium, Was awarded 2nd place for this presentation

• 2009, The Effects of a Natural Anti-Inflammatory Product On Systemic Markers on Inflammation Following Downhill Running, Edith M. Peters, Smith. M, Docrat. A, Nadar. A, Chetty. K, Passmore. J-A.S, Medicine and Science in Sports and Exercise, 41, 278
• 2008, CaMK activation is required for histone hyperacetylation and MEF2A binding at the MEF2 site on the Glut4 gene, James A. H. Smith, Tertius A. Kohn, Kovin A. Chetty and Edward O. Ojuka, American Journal of Physiology Endocrinology & Metabolism, 295, 698-704

• 2013, Proofreader for Siyavula Grade 8 and 9 Life Sciences textbook
• 2012, Volunteer Tutor, Golden Future Project, Tutor high school subjects from five high schools from Khayelitsha, Cape Town, South Africa

• 2019, Paint with Passion Floral Still Life Course, Jenkins Art Studio
• 2012, Diploma in Astrological Studies from the Rod Suskin School of Astrology
• 2007, Music Theory (Grade 5) from the Associated Board of the Royal Schools of Music