Germinah Marole
Veterinary Technologist
Pretoria
Summary
Registered Veterinary Technologist with proven expertise in microbiology quality control and diagnostic laboratory testing. Committed to deliver high quality results in a fast-paced environment.
Overview
6
6
years of professional experience
3
3
Certifications
1
1
Language
Work History
Research and Development Technologist
Design Biologix
Pretoria
08.2023 - Current
- Perform quality control assays for antigens, including ELISA, Western blot, cytotoxicity assay, and enzyme activity tests Prepare cell culture in flasks and maintain viral cells
- Extracting DNA/RNA and perform conventional PCR or QPCR Perform Pichia competent cells, transformation, protein expression and recombinant protein purification
- Perform SDS-PAGE gel electrophoresis and coomassie blue staining
- Perform purification and dialysis of samples
- Analyze assay data, interpret findings and compile reports
- Writing and reviewing standard operating procedures
- Stock taking of lab consumables
Quality Control Technologist
Design Biologix
Pretoria
07.2022 - 08.2023
- Conduct water quality testing for in-house manufacturing plant, by performing membrane filtration, pH, conductivity, resistivity, TOC and endotoxin testing
- Perform sterility testing on raw materials, intermediate, and final vaccine products
- Compile laboratory test reports
- Determine pH, endotoxin, and moisture and formaldehyde level in vaccines
- Perform titrations and viable count
- Prepare culture media, conduct sterility test, and growth promotion of media
- Monitor sterility samples in incubators and conduct environmental monitoring
- Manage retention sample storage
Laboratory Technician
IDEXX Laboratories
Johannesburg
07.2021 - 07.2022
- Manage sample reception and register patient samples in LIMS
- Conduct serological tests, including ELISA and rapid antibody tests
- Send away samples that are not tested in-house to reference laboratories
- Perform blood and urine chemistry laboratory testing using clinical chemistry analyzers
- Generate laboratory tests results report
Work Integrated Learning
Tshwane University of Technology
Pretoria
01.2020 - 12.2020
- I rotated across key laboratory disciplines, including Parasitology, Molecular Biology, Serology and Bacteriology
- Responsible for performing routine diagnostic tests and processing biological samples
- Bacterial isolation and identification using various biochemical tests
- Performing conventional PCR, RTPCR and DNA sequencing Perform serological assays such as ELISA and agglutination Preparation of blood smear, staining, cell identification and manual blood cell count
- Preservation of histological tissues, cutting of tissues, tissue embedding and staining
- Performing parasitology fecal test method such as McMaster, Visser Sieve, flotation, sedimentation and Baermann technique
Education
Bachelor of Life Sciences - Biochemistry And Microbiology
University of South Africa
Pretoria 04.2001 -
National Diploma - Veterinary Technology
Tshwane University of Technology
Pretoria 04.2001 -
Matric -
Mabodibeng Secondary School
Jane Furse 04.2001 -
Skills
Reagent Preparation
Aseptic Technique
Lab Equipment Operation
Written and Verbal Communication
Team Work and Working Independently
LIMS
Certification
Laboratory Management Systems Based on ISO/IEC 17025:2017
Timeline
Research and Development Technologist
Design Biologix
08.2023 - Current
Quality Control Technologist
Design Biologix
07.2022 - 08.2023
Laboratory Technician
IDEXX Laboratories
07.2021 - 07.2022
Work Integrated Learning
Tshwane University of Technology
01.2020 - 12.2020
Bachelor of Life Sciences - Biochemistry And Microbiology
University of South Africa
04.2001 -
National Diploma - Veterinary Technology
Tshwane University of Technology
04.2001 -
Matric -
Mabodibeng Secondary School
04.2001 -